560 million people or 8% of the world population comprising mostly of East Asians
from countries like China, Korea, Taiwan, Japan and Hong Kong are at a higher risk of developing Alzheimer
due to a presence of a mutated gene. The risk is even aggravated by alcohol
According to a new study by researchers at the Stanford University School of Medicine, a common mutation in a key enzyme involved in alcohol
metabolism increases damage in cells from patients with Alzheimer's disease and in mice.
The mutation in aldehyde dehydrogenase 2
, or ALDH2, is typically associated with facial redness following alcohol consumption. It causes the activity of the enzyme to be greatly reduced, resulting in the buildup of acetaldehyde, a toxic product of alcohol metabolism. The body responds to the presence of the toxin with skin flushing and inflammation.
The mutation is prevalent in the East Asian
population. The flushing response to alcohol
among people who carry the mutation is sometimes called "Asian glow
Dr Daria Mochly-Rosen, Ph.D., professor of chemical and systems biology said that understanding the relationship of alcohol
and genes linked to Alzheimer
's disease will have broad consequences, she said, since a large group of people may unknowingly be harming their future health by regularly consuming alcohol.
Dr Mochly-Rosen told Thailand Medical
News, "Our data suggest that alcohol
's disease-prone genes may put humans at greater risk of Alzheimer
's onset and progression. This is based on our patient-derived cell studies and our animal studies, so an epidemiological study in humans should be carried out in the future."
Dr Mochly-Rosen, who holds the George D. Smith Professorship in Translational Medicine, is the senior author of the study, which will be published Dec. 12 in Acta Neuropathologica Communications
. The lead author is postdoctoral scholar Dr Amit Joshi, Ph.D.
Previous epidemiological studies in East Asian
populations have suggested an association between the mutation in ALDH2
that causes facial flushing and Alzheimer
's disease. However, there have also been other studies that didn't find an association. To further explore a possible role for ALDH2
, the authors of the current study examined cell cultures made using cells from 20 patients with Alzheimer'
s disease. One culture had the ALDH2
mutation, also known as ALDH2*2. While the amount of ALDH2*2 protein in this sample matched the level of ALDH2
protein in normal cells, the mutant protein had only a fraction of the ability to break down acetaldehyde.
When compared to normal cells, the ALDH2*2 cells had more free radicals and more 4-HNE, anot
her toxic chemical that's normally processed by ALDH2.
Dr Mochly-Rosen added, "Free radicals are formed when we have fever, when we have chronic diseases, when we are stressed; free radicals are formed under many kinds of pathological stimuli. These free radicals form toxic aldehydes, and the job of ALDH2
is to remove these toxic chemicals. Once these aldehydes accumulate, the first organelles that they damage are the organelles that contain the enzyme that is supposed to get rid of them: the mitochondria. This vicious cycle ultimately leads to reduced mitochondrial activity, increased free radical formation by the damaged mitochondria and, in the case of Alzheimer
's disease, to death of neurons.”
The level of free radicals was restored to normal following the addition of Alda-1, a small molecule that "fixes" ALDH2*2 by binding to the catalytic site and restoring the enzyme to a functional structure.
Dr Mochly-Rosen and her colleagues discovered Alda-1 as an activator of ALDH2*2 in 2008. Alda-1 also activates the nonmutant ALDH2
and therefore may benefit more people, she said. Clinical trials are in progress to test the usefulness of Alda-1-like molecules as a treatment for a variety of health conditions. Mochly-Rosen consults for these clinical trials but does not own stocks in the pharmaceutical company conducting the them. The company did not fund any of the current research.
Upon adding alcohol
to cells with either ALDH2
or ALDH2*2 derived from patients with Alzheimer'
s disease led to an increase in free radicals; the effect was greater, though, in the ALDH2*2 cells. Alda-1 reversed these effects, though not completely. These results indicate that alcohol
damages cells normally protected by ALDH2
and that this damage is more severe in cells from patients with a genetic form of Alzheimer
's disease, the study reports.
To extend the research further understand the link between alcohol
, the researchers studied mice that carry ALDH2*2. The mice were injected with alcohol
each day for 11 weeks to simulate chronic alcohol
Dr Joshi commented, "The animals were given one gram per kilogram per day, which is equivalent to about four to five drinks for the animal. But since mice metabolize alcohol
much faster than humans, it comes to about two drinks a day."
Similar to cell cultures, the researchers saw that mice with the ALDH2*2 gene produced more free radicals than normal mice when provided with alcohol.
The mutant mice also accumulated beta-amyloid protein fragments and activated tau protein more than normal mice. Both of these changes are molecular signatures for Alzheimer
's disease. Treatment with Alda-1 reduced the accumulation of both of these toxic proteins.
Also, the ALDH2*2 mice showed an increase in neuroinflammatory signs following their injection with alcohol
compared with normal mice. Neuroinflammation, or inflammation of the nervous system, is normally caused by injury, infection and even the aging process, but recent studies have found that chronic neuroinflammation worsens the progression of neurodegenerative diseases, such as Alzheimer
's disease. Treatment with Alda-1 reduced the accumulation of these neuroinflammatory proteins in mice.
The medical researchers also prepared cell cultures from the brains of normal and ALDH2*2 mice and found that alcohol
led to increased levels of free radicals and cell-death proteins not only in neurons, but in astrocytes, as well. Astrocytes are cells found in the central nervous system that provide support for neuronal function and maintenance, but that can also contribute to neuroinflammation. Treatment with Alda-1 reduced the alcohol
-induced changes in the cell cultures, the study found.
The study findings point to a previously undiscovered role of alcohol
's disease. Since the work was done in cell cultures and mice, further validation is needed in large epidemiological studies of humans to see whether alcohol
drinkers who have the ALDH2*2 mutation develop Alzheimer'
s disease at a higher-than-average rate, Dr Mochly-Rosen said. Such studies could help determine whether decreased alcohol
consumption and treatment with compounds, such as Alda-1, might reduce the progression and burden of Alzheimer
's disease in the world's aging population.
Further to this, there have also been other studies demonstrating that ALDH2*2 increases the risk of developing cancer in the esophagus. Because ALDH2
is so important for human health, Che-Hong Chen, a senior research scientist in the Mochly-Rosen Lab, organized the Stanford-Taiwan ALDH2
Deficiency Research Consortium, or STAR. This group aims to promote research and public awareness about the ALDH2
mutation in East Asia
, where nearly half the population carries it.
Dr Mochly-Rosen concluded, “Alda-1-like compounds may prove to aid in treatment to reduce the risk of Alzheimer
's disease in humans.”