Researchers From Helsinki University Develop New Platform To Faster Detect Celiac Disease Antibodies
Medical researchers from the University of Helsink
i have developed a new diagnostic method for the rapid on-site measurement of antibodies from patient samples. They have applied this new method for the diagnostics of celiac disease
, with promising results.
Point-of-care (POC) testing is a rapidly growing sector, bringing medical testing from central laboratories to where the patient is receiving care.
Medical researchers from the University of Helsinki
have developed a novel diagnostic method coined RFS or Rapid FRET serodiagnostics
for the rapid on-site measurement of antibodies from patient samples. This technology could revolutionize the serodiagnosis of microbial, autoimmune and allergic disorders.
The team of researchers have applied the new concept for diagnostics of celiac disease
Typically, Celiac disease
is an autoimmune disorder typically presenting as non-specific gastrointestinal symptoms. It affects approximately 1 % of world population, with more than 90 % of patients undiagnosed. Even in affluent countries, more than half of celiacs are unaware of their disease. Delayed diagnosis is associated with impaired quality of life and persistent symptoms even after treatment.
At least one screening of celiac disease
is recommended for patients with e.g. gastrointestinal symptoms or other autoimmune diseases as well as for the first-degree relatives of celiac patients. The screening test involves measurement of disease-specific antibodies from a blood sample.
The medical researchers gathered samples from 70 celiacs at the Kuopio University Hospital, half from children and half from adults. Control samples from healthy donors were also collected. The samples were measured using the new assay and results compared with two currently used methods.
Dr Juuso Rusanen, lead author of the study told Thailand Medical
News, “The performance of the test was comparable to that of current methods. The prevailing method involves transporting the sample to a central laboratory and a multi-step procedure taking hours.
With the new method, results can be achieved in less than half an hour by simply combining the sample and a reagent mix, waiting for a while and reading the result. We hope our rapid method could lower the threshold for screening of celiac disease
and thus help overcome the vast underdiagnosis of this relatively common condition.”
Dr Rusanen further commented, “Additionally, this is the first time the new method has been used for diagnostics of autoimmune disease. This is a promising result, and prompts the development of similar tests for diagnostics of other autoimmune disorders.”
Reference: Juuso Rusanen, Anne Toivonen, Jussi Hepojoki, Satu Hepojoki, Pekka Arikoski, Markku Heikkinen, Outi Vaarala, Jorma Ilonen and Klaus He
dman. LFRET, a novel rapid assay for anti-tissue transglutaminase antibody detection. PLOS One 2019, 14, e0225851; https://doi.org/10.1371/journal.pone.0225851