Contrary To Misinformation, COVID-19 Mortality And Hospitalization Has Been Rising In The Last 30 Days Globally. Please Take Precautions!
Ribavirin, the nucleoside analog 1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide, is a broad spectrum direct antiviral agent. This drug was discovered more than 40 years ago and is efficient both in vitro and in vivo against several RNA or DNA viruses.
Ribavirin represents a water-soluble, guanosine nucleoside analogue that mimics other purines, including inosine and adenosine. Structure-activity relationship studies reveal that the 1,2,4-triazole ring, carboxamide group and the beta-D-ribofuranosyl moiety are pivotal for antiviral activity of this drug.
Ribavirin's base moiety closely resembles the monocyclic base found in nicotinamide and 5-aminoimidazole-4-carboxyamide ribonucleoside, which are both naturally occurring metabolites. Furthermore, its sugar moiety (present as ribose with a hydroxy group at the 2' carbon position) allows ribavirin to be preferentially active in RNA-related metabolism.
Upon absorption, cellular enzymes convert the compound into several metabolites, including the monophosphates, diphosphates and triphosphates, as well as the deribosylated base. These derivatives consequently inhibit viral nucleic acid synthesis, principally by altering the normal formation of messenger RNA.
A plethora of analogues of ribavirin have been synthesized, most notably 3-carboxamidine derivative (found to be similar to ribavirin) and thiocarboxamide, which is active only against DNA viruses. Ribamidine has been shown as highly active against Punta Toro virus in mice and Pichinde virus in hamsters.
The nucleoside antibiotic bredinin is structurally in close relationship with ribavirin. Bredinin and its 5'-phosphate are active against L-1210 cells (mouse lymphocytic leukemia cell line). The mechanism of action of this substance involves the inhibition of inosine monophosphate dehydrogenase.
Different techniques can be employed to produce ribavirin, and among them most commonly used are synthetic, enzymatic and fermentative methods. Chemical synthesis is the most frequently encountered in the current industry. However, because of the troublesome manipulation and the ecologically unfriendly process, this method of production is far from ideal.
In enzymatic method purine nucleoside phosphorylase is used to catalyze the synthesis of ribavirin from its precursors – purine nucleoside and 2H-1,2,4-triazole-3-carboxamide (TCA). Nonetheless, the cost of this technique is high due to the high price of precursors and expensive enzyme source.
Fermentative method was described in 1976 where TCA was added to the culture of nucleoside producing strains of bacterial genera Brevibacterium or Bacillus; ribavirin was subsequently synthesized from endogenous nucleoside catalyzed by the natural purine nucleoside phosphorylase. The main drawback is a low yield of ribavirin.
A combination of traditional fermentation with enzymatic synthesis process was successfully used to establish a novel ribavirin biosynthesis pathway. By overexpressing purine nucleoside phosphorylase, the previous end product purine nucleoside can be successfully converted to ribavirin. Guanosine-producing strain derivate was screened out as the optimal ribavirin-producing strain.