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The aim of gene therapy is the successful integration of corrected, therapeutic DNA into the genomes of cells to alter gene expression and, in turn, protein synthesis to correct genetic diseases.
Viruses are particles made up of an outer protein coat and an inner core of genetic material in the form of DNA or RNA. Viruses do not possess the components needed to multiply or survive on their own and instead depend on a host to replicate.
Viruses may be air-bourne, passed on through sexual contact, skin-to-skin contact or even through sharing drinks and toothbrushes. An outline of viral infection and replication is given below:
Viruses can be modified in the laboratory to provide vectors that carry corrected, therapeutic DNA into cells, where it can be integrated into the genome to alter abnormal gene expression and correct genetic disease.
This involves removing the viral DNA present in the virus and replacing it with the therapeutic genes. In this way, the virus becomes merely a "vector" that is capable of transferring the desired gene into cells but not capable of taking over or harming cells.
Some of the viruses currently used in gene therapy include retroviruses, adenoviruses, adeno-associated viruses and the herpes simplex virus.